Comparative Transcriptome Analysis between Embryogenic and Non-Embryogenic Callus of Davidia involucrata

Davidia involucrata Baill. (D. involucrata), a rare and endangered wild plant, is native to China globally recognized as an ornamental tree species. However, D. exhibits inherent biological characteristics that contribute its low reproductive efficiency. To address this challenge, somatic embryogenesis, biotechnological method, offers numerous advantages, including enhanced efficiency, large coefficient, complete structural composition. Consequently, embryogenesis holds significant value in the propagation genetic improvement of particular In previous study, we utilized immature zygotic embryos explants induced from embryogenic callus, thereby establishing rapid plant regeneration scheme. Illumina RNA sequencing compare transcriptomes callus (EC) non-embryogenic (NEC) involucrata. The analysis revealed 131,109 unigenes assembled EC NEC, 12,806 differentially expressed genes (DEGs) were identified. verify authenticity transcriptome results, qRT-PCR was performed 16 DEGs screened, with stable reference gene UBQ being selected. Our focused on related growth regulators such Aux, IAA, ARF, GH3, AHP, ARR, CYCD, BBM, WUS, GRF, SERK, WOX families. We found certain these families significantly upregulated induction compared indicating they play crucial roles cell proliferation, differentiation, totipotency. These results offer new insights into role EC, may guide efforts improve embryo induction, culture conditions, transformation efficiency